Goat anti-Pig IgG
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.84050-1mg | €27.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.84050-10mg | €1562.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.84050-100mg | €1562.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.84050-100ul | €42.50 |
| Catalog No | CAB.84050 |
|---|---|
| Product Name | Goat anti-Pig IgG |
| Isotype | IgG |
| Calculated MW | 150kDa |
| Immunogen | Pig IgG full length protein |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Pig |
| Application | ELISA WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300（PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Immunoglobulin G (IgG)，is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions;to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Protocol / Instructions
The Goat anti-Pig IgG antibody is a versatile tool for detecting Pig IgG in various applications.
For ELISA, the recommended dilution is 1:5000-100000. The reagents required include the Goat anti-Pig IgG antibody, Pig IgG antigen, ELISA plates, and detection reagents. Sample preparation involves coating the ELISA plates with the Pig IgG antigen. The antibody incubation step involves adding the Goat anti-Pig IgG antibody at the recommended dilution. Washing and detection involve using a conjugated secondary antibody and a chromogenic substrate. Technical notes: Optimize the antibody dilution and incubation time for best results.
For Western Blotting, the recommended dilution is 1:1000-20000. The reagents required include the Goat anti-Pig IgG antibody, SDS-PAGE gel, transfer buffer, and detection reagents. Sample preparation involves separating the proteins by SDS-PAGE and transferring them to a membrane. The antibody incubation step involves adding the Goat anti-Pig IgG antibody at the recommended dilution. Washing and detection involve using a conjugated secondary antibody and a chemiluminescent substrate. Technical notes: Use a suitable blocking buffer to minimize non-specific binding.
Use assay conditions validated for ELISA WB, apply Goat anti-Pig IgG at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB 1:1000-20000.;ELISA:1:5000-100000..
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images