Goat Anti-Bovine IgG/RBITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.3ml | CAB.84153-0.3ml | €47.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.84153-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.84153-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.84153-100ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.84153-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.84153 |
|---|---|
| Product Name | Goat Anti-Bovine IgG/RBITC |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Bovine IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Bovine IgG/RBITC antibody is a valuable tool for various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM).
Reagents or buffers: Phosphate-buffered saline (PBS), Triton X-100 for permeabilization, and a mounting medium with DAPI for nuclear staining.
Sample preparation: Fix cells or tissue sections with paraformaldehyde, followed by permeabilization with Triton X-100.
Antibody incubation: Incubate samples with the Goat Anti-Bovine IgG/RBITC antibody at a dilution of 1:50-500 in PBS for 1-2 hours at room temperature or overnight at 4°C.
Washing or detection: Wash samples extensively with PBS, then mount with a DAPI-containing mounting medium for nuclear staining.
Technical notes: Optimize the antibody dilution and incubation time based on the specific application and sample type.
Reagents or buffers: PBS, FACS buffer (PBS with 1% BSA and 0.1% sodium azide), and a fixation buffer (e.g., paraformaldehyde).
Sample preparation: Prepare cell suspensions and fix cells with paraformaldehyde if necessary.
Antibody incubation: Incubate cells with the Goat Anti-Bovine IgG/RBITC antibody at a dilution of 1:50-500 in FACS buffer for 30 minutes to 1 hour on ice.
Washing or detection: Wash cells extensively with FACS buffer, then analyze by flow cytometry.
Technical notes: Use a compensation control to account for spectral overlap and optimize the antibody dilution based on the specific cell type and application.
Use assay conditions validated for IF, apply Goat Anti-Bovine IgG/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:50-500;
IF=1:50-500.
Use assay conditions validated for FCM, apply Goat Anti-Bovine IgG/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:50-500;
IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, and use appropriate controls. Optimize antibody dilution and incubation conditions to minimize background and ensure specific staining.
Validation Images