Goat anti-Human Fibrinogen
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.84862-1mg | €37.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mg | CAB.84862-5mg | €2500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.84862-10mg | €2500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.84862-100mg | €2500.00 |
| Catalog No | CAB.84862 |
|---|---|
| Product Name | Goat anti-Human Fibrinogen |
| Isotype | IgG |
| Calculated MW | 95/340kDa |
| Immunogen | Human Fibrinogen |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Human |
| Application | ELISA WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300 （PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Fibrinogen is the main protein of blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha，beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A (1 to 16 amino acids) and B (1 to 17 amino acids) are released by thrombin from the N terminal parts of alpha and beta chains，respectively. In this way fibrinogen is converted into fibrin，which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI，thromboembolism and thromboses of arteries and veins，since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation，tumor growth and many other diseases.
Protocol / Instructions
The Goat anti-Human Fibrinogen antibody is designed for use in various applications including ELISA and Western Blotting.
For ELISA, the recommended dilution is 1:1000-100000. Reagents and buffers required include the antigen of interest, a 96-well plate, and an ELISA detection system. Sample preparation involves coating the 96-well plate with the antigen, followed by blocking and washing steps. The antibody is then incubated with the antigen, followed by washing and detection using an enzyme-linked secondary antibody. Technical notes: Optimize the antibody dilution and incubation time to achieve the best results.
For Western Blotting, the recommended dilution is 1:1000-20000. Reagents and buffers required include the sample of interest, SDS-PAGE equipment, and a Western Blotting detection system. Sample preparation involves separating the proteins using SDS-PAGE, followed by transferring the proteins to a membrane. The membrane is then blocked, and the antibody is incubated with the membrane, followed by washing and detection using an enzyme-linked secondary antibody. Technical notes: Use a suitable positive control to confirm the specificity of the antibody.
Use assay conditions validated for ELISA WB, apply Goat anti-Human Fibrinogen at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB 1:1000-20000.;ELISA:1:1000-100000..
Precautions
Store at -20°C. Handle with care, avoid repeated freeze-thaw cycles. Use appropriate controls and optimize antibody dilution for best results.
Validation Images