Goat anti-Human Fibrinogen/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.85210-100ul | €55.00 |
| Catalog No | CAB.85210 |
|---|---|
| Product Name | Goat anti-Human Fibrinogen/HRP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Human Fibrinogen |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
## Introduction
The Goat anti-Human Fibrinogen/HRP antibody is a versatile tool for detecting human fibrinogen in various applications, including Western Blotting (WB), Enzyme-Linked Immunosorbent Assay (ELISA), Immunohistochemistry on paraffin sections (IHC-P), and Immunohistochemistry on frozen sections (IHC-F). This protocol outlines the procedures for each validated application.
For WB, use a dilution of 1:1000 to 1:10000. Prepare samples by lysing cells or tissues in an appropriate buffer, then separate proteins by SDS-PAGE and transfer to a membrane. Block the membrane with 5% milk or BSA in TBS-T, then incubate with the Goat anti-Human Fibrinogen/HRP antibody. CABter washing, detect the signal using an HRP substrate.
For ELISA, use a dilution of 1:1000 to 1:10000. Coat plates with antigen, block with 5% milk or BSA, then incubate with the Goat anti-Human Fibrinogen/HRP antibody. CABter washing, detect the signal using an HRP substrate.
For IHC-P, use a dilution of 1:100 to 1:1000. Prepare paraffin sections by deparaffinization and rehydration. Perform antigen retrieval if necessary, then block with 5% milk or BSA. Incubate with the Goat anti-Human Fibrinogen/HRP antibody, followed by washing and detection using an HRP substrate.
For IHC-F, use a dilution of 1:100 to 1:1000. Prepare frozen sections by fixation and permeabilization. Block with 5% milk or BSA, then incubate with the Goat anti-Human Fibrinogen/HRP antibody. CABter washing, detect the signal using an HRP substrate.
## Technical Notes
Optimize the dilution and incubation time for each application as needed. Use appropriate controls to ensure specificity and sensitivity.
Precautions
Store at 2-8°C. Handle with care, avoid repeated freeze-thaw cycles. Use proper controls and optimize conditions for each application.
Validation Images