Mouse Anti-Bovine IgG/PE
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.85234-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.85234-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.85234-100ul | €200.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.85234-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.85234 |
|---|---|
| Product Name | Mouse Anti-Bovine IgG/PE |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | N/A |
| Reactivity | Bovine IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Mouse Anti-Bovine IgG/PE antibody is a valuable tool for various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM).
For IF, start by preparing your samples, which may include tissue sections or cell cultures. Fix the samples using a suitable fixative such as paraformaldehyde, followed by permeabilization if necessary.
Reagents or buffers required include the Mouse Anti-Bovine IgG/PE antibody, a blocking buffer to reduce non-specific binding, and a mounting medium for preserving the samples CABter staining.
Incubate the samples with the Mouse Anti-Bovine IgG/PE antibody at a dilution of 1:100-1000, depending on the specific requirements of your experiment.
CABter incubation, wash the samples thoroughly to remove unbound antibody, and then proceed with detection.
Technical notes: Optimization of the antibody dilution and incubation time may be necessary to achieve the best results.
For FCM, prepare your cell samples by washing and resuspending them in an appropriate buffer.
Reagents or buffers required include the Mouse Anti-Bovine IgG/PE antibody and a suitable flow cytometry buffer.
Incubate the cells with the Mouse Anti-Bovine IgG/PE antibody at a dilution of 1:100-1000.
CABter incubation, wash the cells to remove unbound antibody, and then analyze using a flow cytometer.
Technical notes: It is crucial to optimize the antibody dilution and incubation time for your specific cell type and experimental conditions to achieve the best results.
Use assay conditions validated for IF, apply Mouse Anti-Bovine IgG/PE at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Mouse Anti-Bovine IgG/PE at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation times for best results.
Validation Images