Donkey Anti-Goat IgG/APC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.86132-100ul | €200.00 |
| Catalog No | CAB.86132 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/APC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/APC is a versatile antibody suitable for various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM). Below are the detailed protocols for each validated application.
For IF, the Donkey Anti-Goat IgG/APC can be used to detect goat IgG in various samples. Reagents needed include the antibody, mounting medium, and fluorescent dyes if necessary. Sample preparation involves fixing and permeabilizing cells or tissues. The antibody is then incubated with the sample at a dilution of 1:100-1000 for 1-2 hours at room temperature or overnight at 4°C. CABter washing, the sample can be mounted and visualized under a fluorescence microscope. Technical notes: Ensure proper fixation and permeabilization to maintain sample integrity and optimize fluorescence signal.
In FCM, the Donkey Anti-Goat IgG/APC is utilized to detect and analyze goat IgG on the surface of cells. Required reagents include the antibody, FCM buffer, and potentially cell surface markers. Sample preparation involves suspending cells in FCM buffer. The antibody is then added at a dilution of 1:100-1000 and incubated for 15-30 minutes on ice. CABter washing, cells are resuspended in FCM buffer and analyzed using a flow cytometer. Technical notes: Optimize antibody dilution and incubation time for specific cell types and experimental conditions to minimize background and enhance signal.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Donkey Anti-Goat IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation conditions for best results.
Validation Images