Goat anti-Human IgG
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.87268-1mg | €27.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.87268-10mg | €1562.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.87268-100mg | €1562.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.87268-100ul | €150.00 |
| Catalog No | CAB.87268 |
|---|---|
| Product Name | Goat anti-Human IgG |
| Isotype | IgG |
| Calculated MW | 150kDa |
| Immunogen | Human IgG full length protein |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Human |
| Application | WB;ELISA |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300（PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Immunoglobulin G (IgG)，is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions;to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Protocol / Instructions
The Goat anti-Human IgG antibody is designed for the detection of human IgG in various applications.
For Western blotting, the recommended dilution is 1:1000-20000. The reagents required include the antibody, blocking buffer, and washing buffer. Sample preparation involves separating the proteins by SDS-PAGE and transferring them to a membrane. The membrane is then blocked with blocking buffer to reduce non-specific binding. The antibody is incubated with the membrane for 1-2 hours, followed by washing with washing buffer to remove excess antibody. Detection is achieved using a secondary antibody conjugated to an enzyme or fluorophore. Technical notes: the optimal dilution and incubation time may vary depending on the specific application and sample.
For ELISA, the recommended dilution is 1:5000-200000. The reagents required include the antibody, coating buffer, blocking buffer, and substrate. Sample preparation involves coating the plate with the antigen and blocking with blocking buffer. The antibody is incubated with the antigen for 1-2 hours, followed by washing with washing buffer to remove excess antibody. Detection is achieved using a secondary antibody conjugated to an enzyme and a substrate. Technical notes: the optimal dilution and incubation time may vary depending on the specific application and sample.
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images