Donkey Anti-Goat IgG/Gold 15nm
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
500ul | CAB.88230-500ul | €42.50 |
| Catalog No | CAB.88230 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Gold 15nm |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | GICA;IGS;IEM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Store at 2-8℃ for 3-6 months. Avoid repeated freeze/thaw cycles. |
Background
The particle size is 15nm
Protocol / Instructions
The Donkey Anti-Goat IgG/Gold 15nm antibody is a versatile reagent for various immunocytochemical and immunogold silver staining techniques.
It is recommended for use in Grid Immunogold Silver Staining (GICA), Immunogold Silver Staining (IGS), and Immunoelectron Microscopy (IEM) applications.
Reagents or buffers: 0.01M phosphate buffer, pH 7.4, 1% bovine serum albumin, Donkey Anti-Goat IgG/Gold 15nm.
Sample preparation: Prepare tissue sections or cell samples according to standard protocols.
Antibody incubation: Incubate samples with Donkey Anti-Goat IgG/Gold 15nm at a dilution of 1:20-200 for 1-2 hours at room temperature.
Washing or detection: Wash samples thoroughly with 0.01M phosphate buffer, then proceed with silver enhancement according to the manufacturer's instructions.
Technical notes: Optimize the antibody dilution and incubation time for specific applications.
Reagents or buffers: 0.01M phosphate buffer, pH 7.4, 1% bovine serum albumin, Donkey Anti-Goat IgG/Gold 15nm.
Sample preparation: Prepare tissue sections or cell samples according to standard protocols.
Antibody incubation: Incubate samples with Donkey Anti-Goat IgG/Gold 15nm at a dilution of 1:20-200 for 1-2 hours at room temperature.
Washing or detection: Wash samples thoroughly with 0.01M phosphate buffer, then proceed with silver enhancement according to the manufacturer's instructions.
Technical notes: Optimize the antibody dilution and incubation time for specific applications.
Reagents or buffers: 0.01M phosphate buffer, pH 7.4, 1% bovine serum albumin, Donkey Anti-Goat IgG/Gold 15nm.
Sample preparation: Prepare tissue sections or cell samples according to standard protocols for electron microscopy.
Antibody incubation: Incubate samples with Donkey Anti-Goat IgG/Gold 15nm at a dilution of 1:20-200 for 1-2 hours at room temperature.
Washing or detection: Wash samples thoroughly with 0.01M phosphate buffer, then proceed with standard electron microscopy protocols.
Technical notes: Optimize the antibody dilution and incubation time for specific applications.
General considerations: Handle the antibody with care, and store at -20°C to maintain activity.
Precautions
Store at -20°C, handle with care, optimize antibody dilution and incubation time for specific applications, and use appropriate controls to ensure specificity and sensitivity.
Validation Images