Donkey Anti-Human IgG/Gold
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
500ul | CAB.88373-500ul | €75.00 |
| Catalog No | CAB.88373 |
|---|---|
| Product Name | Donkey Anti-Human IgG/Gold |
| Isotype | N/A |
| Calculated MW | 160kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Human IgG |
| Application | ICA;IEM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Store at 2-8℃ for 3-6 months. Avoid repeated freeze/thaw cycles. |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Human IgG/Gold antibody is designed for use in various immunocytochemical assays.
For ICA, start by preparing the sample. Fix the cells with a suitable fixative, such as paraformaldehyde, and permeabilize with a detergent like Triton X-100.
Use a blocking buffer to reduce non-specific binding, then incubate the sample with the primary antibody.
The Donkey Anti-Human IgG/Gold can be used at a dilution of 1:20-200.
CABter primary antibody incubation, wash the sample with a suitable washing buffer.
Technical notes: Optimization of the antibody dilution and incubation time may be necessary for optimal results.
For IEM, prepare the sample by fixing with a suitable fixative, such as glutaraldehyde, and then embed the sample in a resin.
Section the sample into thin slices and mount on grids.
Use the Donkey Anti-Human IgG/Gold at a dilution of 1:20-200.
CABter incubation with the antibody, wash the grids with a suitable washing buffer.
Technical notes: The choice of resin and sectioning technique may CABfect the outcome of the IEM experiment.
General technical notes: The antibody should be handled and stored according to the manufacturer's instructions to ensure optimal performance.
Use assay conditions validated for ICA, apply Donkey Anti-Human IgG/Gold at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: ICA=1:20-200;
IEM=1:20-200.
Use assay conditions validated for IEM, apply Donkey Anti-Human IgG/Gold at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: ICA=1:20-200;
IEM=1:20-200.
Precautions
Store at 2-8°C. Handle with care, avoid freeze-thaw cycles, and optimize antibody dilution for each application to minimize non-specific binding.
Validation Images