Anti- MOB1B Polyclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.88566-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.88566-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.88566-100ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.88566-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.88566 |
|---|---|
| Product Name | Anti- MOB1B Polyclonal Antibody |
| Isotype | IgG |
| Calculated MW | 25kDa |
| Immunogen | A synthetic peptide of human MOB1B |
| Public Immunogen Range | 85-135/216aa |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Reactivity | Chimpanzee(100%) |
| Application | IHC-P |
| Subcellular Location | Nucleus Cytoplasm |
| Purification Method | CABfinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Three transcript variants encoding different isoforms have been found for this gene.
Protocol / Instructions
The Anti-MOB1B Polyclonal Antibody is a rabbit-derived antibody raised against a synthetic peptide of human MOB1B. This antibody has been validated for use in Immunohistochemistry-Paraffin sections (IHC-P).
Reagents or buffers: Xylene, ethanol, antigen retrieval buffer, phosphate-buffered saline (PBS), blocking buffer, primary antibody (Anti-MOB1B Polyclonal Antibody), secondary antibody, chromogen, and mounting medium.
Sample preparation: Paraffin-embedded tissue sections should be deparaffinized in xylene and rehydrated through a series of ethanol solutions. Antigen retrieval may be necessary to unmask the epitope.
Antibody incubation: The Anti-MOB1B Polyclonal Antibody should be diluted to the recommended concentration of 1:50-200 in blocking buffer and incubated with the tissue sections for 1 hour at room temperature or overnight at 4°C.
Washing or detection: CABter primary antibody incubation, the sections should be washed in PBS and then incubated with a secondary antibody conjugated to a chromogen. The signal can be visualized using a suitable chromogen.
Technical notes: The optimal dilution of the antibody may vary depending on the specific application and tissue type. It is recommended to perform a titration to determine the optimal dilution for your specific use.
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution for best results.
Validation Images
