Donkey Anti-Goat IgG/PE-Cy7
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.88809-100ul | €200.00 |
| Catalog No | CAB.88809 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/PE-Cy7 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/PE-Cy7 antibody is a versatile reagent for various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM). Below are the protocols for each validated application.
Reagents or Buffers: Donkey Anti-Goat IgG/PE-Cy7 antibody, phosphate-buffered saline (PBS), mounting medium.
Sample Preparation: Fix cells or tissue sections with a suitable fixative, permeabilize if necessary, and block non-specific binding sites.
Antibody Incubation: Dilute the Donkey Anti-Goat IgG/PE-Cy7 antibody to 1:100-1000 in PBS and incubate with the sample for 1-2 hours at room temperature or overnight at 4°C.
Washing or Detection: Wash the sample thoroughly with PBS, then mount with a suitable mounting medium and analyze using a fluorescence microscope.
Technical Notes: Optimize the antibody dilution and incubation time for specific applications.
Reagents or Buffers: Donkey Anti-Goat IgG/PE-Cy7 antibody, phosphate-buffered saline (PBS), FCM buffer.
Sample Preparation: Prepare cell suspensions and block non-specific binding sites if necessary.
Antibody Incubation: Dilute the Donkey Anti-Goat IgG/PE-Cy7 antibody to 1:100-1000 in FCM buffer and incubate with the cell suspension for 15-30 minutes at room temperature.
Washing or Detection: Wash the cells with FCM buffer, then analyze using a flow cytometer.
Technical Notes: Optimize the antibody dilution and incubation time for specific cell types and experimental conditions.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/PE-Cy7 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Donkey Anti-Goat IgG/PE-Cy7 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation conditions for specific applications.
Validation Images