Goat anti-Human Albumin /Bio

The Goat anti-Human Albumin antibody is designed for various applications including Western Blotting (WB), Enzyme-Linked Immunosorbent Assay (ELISA), Immunohistochemistry on Paraffin sections (IHC-P), Immunohistochemistry on Frozen sections (IHC-F), and Immunofluorescence (IF).

For WB, prepare samples by lysing cells or tissues in an appropriate buffer, then separate proteins by gel electrophoresis and transfer to a membrane. Block the membrane with 5% milk or BSA in TBS-T, then incubate with the Goat anti-Human Albumin antibody at a dilution of 1:1000-10000 in blocking buffer for 1-2 hours at room temperature. Wash the membrane with TBS-T, and detect using a secondary antibody conjugated to a detection system such as HRP or fluorescent dyes.

For ELISA, coat plates with antigen, block with 5% milk or BSA in PBS, and incubate with the Goat anti-Human Albumin antibody at a dilution of 1:1000-10000. Use a secondary antibody conjugated to an enzyme such as HRP for detection, with a substrate that produces a measurable signal.

For IHC-P, deparaffinize and rehydrate tissue sections, then block endogenous peroxidase and incubate with the Goat anti-Human Albumin antibody at a dilution of 1:100-1000. Use a secondary antibody conjugated to a detection system such as HRP or alkaline phosphatase for visualization.

For IHC-F, fix and permeabilize frozen tissue sections, then block with 5% milk or BSA in PBS and incubate with the Goat anti-Human Albumin antibody at a dilution of 1:100-1000. Detect using a secondary antibody conjugated to a fluorescent dye.

For IF, fix and permeabilize cells or tissue sections, then block with 5% milk or BSA in PBS and incubate with the Goat anti-Human Albumin antibody at a dilution of 1:100-1000. Detect using a secondary antibody conjugated to a fluorescent dye. Technical notes: Optimize antibody dilutions and incubation times for each specific application to achieve the best results.

Use assay conditions validated for IF, apply Goat anti-Human Albumin /Bio at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB=1:1000-10000;

ELISA=1:1000-10000;

IHC-P=1:100-1000;

IHC-F=1:100-1000;

IF=1:100-1000.