BSA/Solar Fluor 405
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.89324-100ul | €87.50 |
| Catalog No | CAB.89324 |
|---|---|
| Product Name | BSA/Solar Fluor 405 |
| Isotype | N/A |
| Calculated MW | 66kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | BSA |
| Clone Type | N/A |
| Reactivity | BSA |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
## Introduction
The BSA/Solar Fluor 405 antibody is a valuable tool for detecting Bovine Serum Albumin (BSA) in various applications. This protocol outlines the steps for using this antibody in Immunofluorescence (IF) applications.
For IF applications, the BSA/Solar Fluor 405 antibody can be used to detect BSA in fixed cells or tissues. The recommended dilution for IF is 1:50-500.
Reagents or buffers required include the BSA/Solar Fluor 405 antibody, a suitable fixation method (e.g., paraformaldehyde), a permeabilization agent (e.g., Triton X-100), and a mounting medium.
Sample preparation involves fixing and permeabilizing the cells or tissues, followed by blocking with a suitable blocking buffer.
Antibody incubation is typically performed at 4°C overnight, using the recommended dilution of the BSA/Solar Fluor 405 antibody.
Washing is done with a suitable washing buffer, and detection can be performed using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time for the specific application and sample type.
Use assay conditions validated for IF, apply BSA/Solar Fluor 405 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images