Donkey Anti-Goat IgG/Solar Fluor 680
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.89378-100ul | €87.50 |
| Catalog No | CAB.89378 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Solar Fluor 680 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Solar Fluor 680 is a secondary antibody used for detecting goat IgG primary antibodies. It is conjugated to Solar Fluor 680, a fluorescent dye that emits at 698 nm.
Reagents or buffers: phosphate-buffered saline (PBS), 4% paraformaldehyde, 0.1% Triton X-100, and a blocking buffer.
Sample preparation: Fix cells or tissue sections with 4% paraformaldehyde, followed by permeabilization with 0.1% Triton X-100.
Antibody incubation: Incubate samples with the primary goat IgG antibody, followed by the Donkey Anti-Goat IgG/Solar Fluor 680 secondary antibody at a dilution of 1:50-500.
Washing or detection: Wash samples with PBS, and detect the fluorescence signal using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time to achieve the best signal-to-noise ratio.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Solar Fluor 680 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 4°C, handle with care, and optimize antibody dilution for best results.
Validation Images