Mouse Anti-Bovine IgG/AP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
25T | CAB.92164-25T | €407.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100T | CAB.92164-100T | €970.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.92164-100ul | €267.50 |
| Catalog No | CAB.92164 |
|---|---|
| Product Name | Mouse Anti-Bovine IgG/AP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | N/A |
| Reactivity | Bovine IgG |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Mouse Anti-Bovine IgG/AP antibody is a versatile tool for detecting bovine IgG in various applications.
For Western blotting, prepare samples by separating proteins using SDS-PAGE and transferring them to a membrane. Block the membrane with 5% non-fat milk in TBS-T for 1 hour. Incubate the membrane with the Mouse Anti-Bovine IgG/AP antibody at a dilution of 1:1000-10000 in TBS-T for 1 hour. Wash the membrane three times with TBS-T and detect using an alkaline phosphatase substrate.
For immunohistochemistry on paraffin-embedded tissues, deparaffinize and rehydrate tissue sections. Block endogenous peroxidase activity and incubate with the Mouse Anti-Bovine IgG/AP antibody at a dilution of 1:100-1000 for 30 minutes. Use a biotinylated secondary antibody and an avidin-biotin complex for detection.
For immunohistochemistry on frozen tissues, fix tissue sections with acetone and incubate with the Mouse Anti-Bovine IgG/AP antibody at a dilution of 1:100-1000 for 30 minutes. Use a fluorescently labeled secondary antibody for detection.
For ELISA, coat plates with bovine IgG and block with 5% non-fat milk in PBS. Incubate with the Mouse Anti-Bovine IgG/AP antibody at a dilution of 1:1000-10000 for 1 hour. Detect using an alkaline phosphatase substrate.
Technical notes: Optimize antibody dilutions and incubation times for each application to achieve the best results.
Precautions
Store at 2-8°C. Handle with care, avoid freeze-thaw cycles. Use appropriate controls and optimize antibody dilutions for each application.
Validation Images