Goat Anti-Human IgG/HRP

The Goat Anti-Human IgG/HRP antibody is a versatile tool for detecting human IgG in various applications. The following protocols are recommended for each validated application.

Reagents or buffers: Tris-buffered saline with Tween 20 (TBST), skim milk or BSA for blocking, and a suitable substrate for HRP.

Sample preparation: Separate proteins by SDS-PAGE and transfer to a membrane.

Antibody incubation: Incubate the membrane with the Goat Anti-Human IgG/HRP antibody at a dilution of 1:1000-10000 in TBST with 5% skim milk or BSA for 1-2 hours at room temperature.

Washing or detection: Wash the membrane three times with TBST, then detect using a suitable HRP substrate.

Technical notes: Optimize the antibody dilution and incubation time for specific experimental conditions.

Reagents or buffers: Phosphate-buffered saline (PBS), normal goat serum or BSA for blocking, a suitable chromogen, and a peroxidase substrate.

Sample preparation: Fix and embed tissue samples, then section and mount on slides.

Antibody incubation: Incubate the sections with the Goat Anti-Human IgG/HRP antibody at a dilution of 1:100-1000 in PBS with 1% normal goat serum or BSA for 30 minutes to 1 hour at room temperature.

Washing or detection: Wash the sections with PBS, then detect using a suitable chromogen and peroxidase substrate.

Technical notes: Perform antigen retrieval if necessary, and optimize the antibody dilution and incubation time for specific tissue types.

Reagents or buffers: Phosphate-buffered saline (PBS), normal goat serum or BSA for blocking, and a suitable fluorescent secondary antibody.

Sample preparation: Fix and permeabilize cells or tissue samples, then block with normal goat serum or BSA.

Antibody incubation: Incubate the samples with the Goat Anti-Human IgG/HRP antibody at a dilution of 1:100-1000 in PBS with 1% normal goat serum or BSA for 30 minutes to 1 hour at room temperature.

Washing or detection: Wash the samples with PBS, then detect using a suitable fluorescent secondary antibody.

Technical notes: Optimize the antibody dilution and incubation time for specific cell or tissue types, and consider using a directly conjugated fluorescent antibody for simpler protocols.

Reagents or buffers: Coating buffer, blocking buffer, and a suitable substrate for HRP.

Sample preparation: Coat ELISA plates with the antigen of interest, then block with a suitable blocking buffer.

Antibody incubation: Incubate the plates with the Goat Anti-Human IgG/HRP antibody at a dilution of 1:1000-10000 in the blocking buffer for 1-2 hours at room temperature.

Washing or detection: Wash the plates with PBS or a suitable washing buffer, then detect using a suitable HRP substrate.

Technical notes: Optimize the antibody dilution and incubation time for specific experimental conditions, and consider using a standard curve to quantify the results.