Donkey Anti-Human IgG/RBITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.93730-100ul | €75.00 |
| Catalog No | CAB.93730 |
|---|---|
| Product Name | Donkey Anti-Human IgG/RBITC |
| Isotype | N/A |
| Calculated MW | 160kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Human IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Human IgG/RBITC is a secondary antibody used for detecting human IgG in various applications. Here are the protocols for the validated application:
For IF, the following reagents and steps are recommended:
- Reagents: Donkey Anti-Human IgG/RBITC, PBS, mounting medium.
- Sample Preparation: Fix and permeabilize cells or tissue sections according to standard protocols.
- Antibody Incubation: Incubate samples with primary antibody followed by the Donkey Anti-Human IgG/RBITC at a dilution of 1:100-1000 for 1 hour at room temperature.
- Washing or Detection: Wash samples thoroughly with PBS and mount with a mounting medium.
- Technical Notes: Optimize the dilution of the secondary antibody for best results.
## Technical Notes
The Donkey Anti-Human IgG/RBITC is conjugated to RBITC, which has an excitation maximum at 550 nm and an emission maximum at 570 nm. This makes it suitable for use in fluorescence microscopy.
Use assay conditions validated for IF, apply Donkey Anti-Human IgG/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution for best results.
Validation Images