Goat anti-Equine IgG
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.94027-1mg | €27.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.94027-10mg | €1562.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.94027-100mg | €1562.50 |
| Catalog No | CAB.94027 |
|---|---|
| Product Name | Goat anti-Equine IgG |
| Isotype | IgG |
| Calculated MW | 150kDa |
| Immunogen | Equine IgG full length protein |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Equine |
| Application | ELISA WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300 （PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Immunoglobulin G (IgG)，is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions;to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Protocol / Instructions
The Goat anti-Equine IgG antibody is designed for use in various applications including ELISA and Western Blotting.
For ELISA, the recommended dilution is 1:5000-50000. Reagents and buffers required include a 96-well plate, antigen, blocking buffer, and substrate. Sample preparation involves coating the plate with antigen and blocking non-specific binding sites. Antibody incubation is performed by adding the Goat anti-Equine IgG antibody at the recommended dilution, followed by washing and detection using a secondary antibody conjugated to an enzyme. Technical notes: Optimize the antibody dilution and incubation time to achieve the best results.
For Western Blotting, the recommended dilution is 1:200-1000. Reagents and buffers required include a transfer membrane, blocking buffer, and substrate. Sample preparation involves separating proteins by SDS-PAGE and transferring them to a membrane. Antibody incubation is performed by adding the Goat anti-Equine IgG antibody at the recommended dilution, followed by washing and detection using a secondary antibody conjugated to an enzyme. Technical notes: Use a suitable positive control to verify the specificity of the antibody and optimize the blocking conditions to reduce non-specific binding.
Use assay conditions validated for ELISA WB, apply Goat anti-Equine IgG at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB 1:200-1000.;ELISA:1:5000-50000..
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images