BSA/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.1ml | CAB.94399-0.1ml | €37.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1ml | CAB.94399-1ml | €250.00 |
| Catalog No | CAB.94399 |
|---|---|
| Product Name | BSA/HRP |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | BSA |
| Clone Type | N/A |
| Reactivity | BSA |
| Application | ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The BSA/HRP antibody is a valuable tool for detecting Bovine Serum Albumin (BSA) in various applications. Below are the protocols for the validated applications.
For ELISA, the following steps are recommended:
Reagents or buffers: TBS, Tween-20, blocking buffer (e.g., 5% non-fat dry milk in TBS), and substrate for HRP.
Sample preparation: Coat the plate with BSA, block with blocking buffer, and prepare the sample.
Antibody incubation: Dilute the BSA/HRP antibody 1:1000-10000 in blocking buffer and incubate for 1 hour at room temperature.
Washing or detection: Wash the plate with TBS-Tween-20, add substrate, and measure the absorbance.
Technical notes: Optimize the antibody dilution and incubation time for best results.
For Western Blotting, the following steps are recommended:
Reagents or buffers: Transfer buffer, blocking buffer (e.g., 5% non-fat dry milk in TBS), and substrate for HRP.
Sample preparation: Prepare the sample, separate the proteins by SDS-PAGE, and transfer to a membrane.
Antibody incubation: Dilute the BSA/HRP antibody 1:1000-10000 in blocking buffer and incubate for 1 hour at room temperature.
Washing or detection: Wash the membrane with TBS-Tween-20, add substrate, and detect the signal.
Technical notes: Optimize the antibody dilution, incubation time, and membrane blocking for best results.
Precautions
Store at 2-8°C, handle with care, use proper controls, and optimize antibody dilution and incubation time for best results.
Validation Images