Donkey Anti-Goat IgG/FITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.95703-100ul | €27.50 |
| Catalog No | CAB.95703 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/FITC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/FITC antibody is designed for use in various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM). The following protocols are recommended for optimal results.
For IF, the recommended dilution is 1:50-500. Reagents needed include the Donkey Anti-Goat IgG/FITC antibody, a suitable mounting medium, and a fluorescence microscope. Sample preparation involves fixing and permeabilizing cells or tissue sections. The antibody is then incubated with the sample for 1-2 hours at room temperature or overnight at 4°C. CABter washing with a suitable buffer, the sample is mounted and visualized using a fluorescence microscope. Technical notes: Use a secondary antibody control to verify specificity.
For FCM, the recommended dilution is 1:50-500. Reagents needed include the Donkey Anti-Goat IgG/FITC antibody and a suitable flow cytometry buffer. Sample preparation involves preparing a single-cell suspension. The antibody is then incubated with the cells for 30 minutes to 1 hour on ice. CABter washing with a suitable buffer, the cells are analyzed using a flow cytometer. Technical notes: Use a secondary antibody control to verify specificity and optimize the dilution for optimal signal-to-noise ratio.
Both protocols require optimization of the antibody dilution and incubation conditions to achieve the best results. It is also crucial to include proper controls to verify the specificity of the antibody.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/FITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:50-500;
IF=1:50-500.
Use assay conditions validated for FCM, apply Donkey Anti-Goat IgG/FITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:50-500;
IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution for each application to avoid non-specific binding.
Validation Images