Goat anti-Equine IgG/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.1ml | CAB.96176-0.1ml | €35.00 |
| Catalog No | CAB.96176 |
|---|---|
| Product Name | Goat anti-Equine IgG/HRP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Equine IgG |
| Application | IHC-F;ELISA;IHC-P;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat anti-Equine IgG/HRP antibody is a versatile tool for detecting Equine IgG in various applications.
For Western blotting, prepare samples by lysing cells or tissues in an appropriate buffer, then separate proteins by SDS-PAGE and transfer to a membrane. Block the membrane with 5% milk or BSA in TBS-T, then incubate with the Goat anti-Equine IgG/HRP antibody at a dilution of 1:1000-10000 in TBS-T. Wash the membrane with TBS-T, and detect using a suitable HRP substrate.
For ELISA, coat plates with antigen, then block with 5% milk or BSA in PBS. Incubate with the Goat anti-Equine IgG/HRP antibody at a dilution of 1:1000-10000 in PBS, followed by washing with PBS-T. Detect using a suitable HRP substrate.
For IHC-P, prepare tissue sections and deparaffinize, then block with 5% milk or BSA in TBS. Incubate with the Goat anti-Equine IgG/HRP antibody at a dilution of 1:100-1000 in TBS, followed by washing with TBS. Detect using a suitable HRP substrate.
For IHC-F, prepare tissue sections and fix, then block with 5% milk or BSA in PBS. Incubate with the Goat anti-Equine IgG/HRP antibody at a dilution of 1:100-1000 in PBS, followed by washing with PBS. Detect using a suitable fluorescence substrate.
Technical notes: Optimize antibody dilutions and incubation times for each specific application to achieve the best results.
Precautions
Store at 2-8°C. Handle with care, and use appropriate controls. Optimize antibody dilutions and incubation times to minimize background and ensure specificity.
Validation Images