Donkey Anti-Guinea Pig IgG/Bio

The Donkey Anti-Guinea Pig IgG/Bio antibody is a versatile tool for various immunological applications. Below are the protocols for each validated application.

Reagents or buffers: Tris-buffered saline with Tween 20 (TBST), skim milk, and ECL substrate.

Sample preparation: Separate proteins by SDS-PAGE and transfer to a PVDF membrane.

Antibody incubation: Incubate the membrane with the Donkey Anti-Guinea Pig IgG/Bio antibody at a dilution of 1:1000-10000 in TBST with 5% skim milk for 1 hour at room temperature.

Washing or detection: Wash the membrane three times with TBST and detect using an ECL substrate.

Technical notes: Optimize the blocking conditions and antibody dilution to minimize background.

Reagents or buffers: Xylene, ethanol, antigen retrieval buffer, and DAB substrate.

Sample preparation: Fix and embed tissue samples, then section and mount on slides.

Antibody incubation: Incubate the sections with the Donkey Anti-Guinea Pig IgG/Bio antibody at a dilution of 1:100-500 in antibody diluent for 30 minutes at room temperature.

Washing or detection: Wash the sections with PBS and detect using a DAB substrate.

Technical notes: Perform antigen retrieval to enhance staining.

Reagents or buffers: Methanol, acetone, and fluorescent mounting medium.

Sample preparation: Fix and permeabilize cells or tissue samples.

Antibody incubation: Incubate the samples with the Donkey Anti-Guinea Pig IgG/Bio antibody at a dilution of 1:100-1000 in antibody diluent for 30 minutes at room temperature.

Washing or detection: Wash the samples with PBS and detect using a fluorescent microscope.

Technical notes: Use a suitable fluorescent secondary antibody for detection.

Reagents or buffers: Methanol, acetone, and fluorescent mounting medium.

Sample preparation: Fix and permeabilize cells or tissue samples.

Antibody incubation: Incubate the samples with the Donkey Anti-Guinea Pig IgG/Bio antibody at a dilution of 1:100-1000 in antibody diluent for 30 minutes at room temperature.

Washing or detection: Wash the samples with PBS and detect using a fluorescent microscope.

Technical notes: Use a suitable fluorescent secondary antibody for detection.

Reagents or buffers: PBS, FACS buffer, and propidium iodide.

Sample preparation: Prepare single-cell suspensions and fix if necessary.

Antibody incubation: Incubate the cells with the Donkey Anti-Guinea Pig IgG/Bio antibody at a dilution of 1:100-1000 in FACS buffer for 30 minutes at 4°C.

Washing or detection: Wash the cells with FACS buffer and detect using a flow cytometer.

Technical notes: Use a suitable fluorescent secondary antibody for detection.

Reagents or buffers: Coating buffer, blocking buffer, and TMB substrate.

Sample preparation: Coat the plate with antigen and block with blocking buffer.

Antibody incubation: Incubate the plate with the Donkey Anti-Guinea Pig IgG/Bio antibody at a dilution of 1:1000-10000 in diluent for 1 hour at room temperature.

Washing or detection: Wash the plate with washing buffer and detect using a TMB substrate.

Technical notes: Optimize the coating and blocking conditions to minimize background.