Goat Anti-Guinea Pig IgG/RBITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.97097-100ul | €100.00 |
| Catalog No | CAB.97097 |
|---|---|
| Product Name | Goat Anti-Guinea Pig IgG/RBITC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Guinea Pig IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Guinea Pig IgG/RBITC antibody is designed for use in various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM).
Reagents or buffers: Phosphate-buffered saline (PBS), mounting medium, and fluorescence microscope.
Sample preparation: Fix and permeabilize cells or tissue sections according to standard protocols.
Antibody incubation: Dilute the antibody to the recommended concentration (1:100-1000) and incubate for 1-2 hours at room temperature or overnight at 4°C.
Washing or detection: Wash samples with PBS and detect using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time for specific applications.
Reagents or buffers: PBS, flow cytometry buffer, and flow cytometer.
Sample preparation: Prepare cell suspensions according to standard protocols.
Antibody incubation: Dilute the antibody to the recommended concentration (1:100-1000) and incubate for 30 minutes to 1 hour at room temperature.
Washing or detection: Wash cells with flow cytometry buffer and detect using a flow cytometer.
Technical notes: Optimize the antibody dilution and incubation time for specific cell types and flow cytometers.
Use assay conditions validated for IF, apply Goat Anti-Guinea Pig IgG/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:100-1000;
FCM=1:100-1000.
Use assay conditions validated for FCM, apply Goat Anti-Guinea Pig IgG/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:100-1000;
FCM=1:100-1000.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation time for specific applications.
Validation Images