Donkey Anti-Goat IgG/Solar Fluor 568
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.97584-100ul | €87.50 |
| Catalog No | CAB.97584 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Solar Fluor 568 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Solar Fluor 568 is a secondary antibody used for detecting goat IgG primary antibodies.
For IF, the following steps are recommended:
- Reagents or buffers: phosphate-buffered saline (PBS), 4% paraformaldehyde for fixation, and a blocking buffer (e.g., 5% normal donkey serum in PBS).
- Sample preparation: Fix and permeabilize cells or tissue sections with 4% paraformaldehyde, followed by washing with PBS.
- Antibody incubation: Incubate samples with the primary goat IgG antibody, then with the Donkey Anti-Goat IgG/Solar Fluor 568 secondary antibody at a dilution of 1:50-500 in blocking buffer for 1-2 hours at room temperature or overnight at 4°C.
- Washing or detection: Wash samples extensively with PBS, then mount with a fluorescence-compatible mounting medium.
- Technical notes: Optimize the dilution of the secondary antibody and the incubation time based on the specific application and the intensity of the fluorescence signal.
## Notes on Other Potential Applications
Although not explicitly validated for other applications, secondary antibodies like Donkey Anti-Goat IgG/Solar Fluor 568 can be explored for other immunofluorescence-based techniques with appropriate optimization and validation.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Solar Fluor 568 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 4°C, handle with care, and optimize antibody dilution for specific applications to minimize background and ensure specific staining.
Validation Images