Donkey Anti-Human IgG/APC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20T | CAB.98199-20T | €115.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100T | CAB.98199-100T | €355.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.98199-100ul | €200.00 |
| Catalog No | CAB.98199 |
|---|---|
| Product Name | Donkey Anti-Human IgG/APC |
| Isotype | N/A |
| Calculated MW | 160kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Human IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Human IgG/APC is a versatile antibody suitable for various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM).
Reagents or buffers: phosphate-buffered saline (PBS), 4% paraformaldehyde, 0.1% Triton X-100, and a mounting medium with DAPI.
Sample preparation: fix cells with 4% paraformaldehyde, permeabilize with 0.1% Triton X-100, and block with 5% normal donkey serum.
Antibody incubation: incubate with the Donkey Anti-Human IgG/APC at a dilution of 1:100-1000 in PBS for 1 hour at room temperature.
Washing or detection: wash with PBS and detect using a fluorescence microscope.
Technical notes: optimize the antibody dilution and incubation time for specific experimental conditions.
Reagents or buffers: PBS, 1% bovine serum albumin (BSA), and a fixation buffer.
Sample preparation: collect cells, wash with PBS, and block with 1% BSA.
Antibody incubation: incubate with the Donkey Anti-Human IgG/APC at a dilution of 1:100-1000 in PBS for 30 minutes on ice.
Washing or detection: wash with PBS and detect using a flow cytometer.
Technical notes: use a compensation control to account for spectral overlap and optimize the antibody dilution for specific experimental conditions.
Use assay conditions validated for IF, apply Donkey Anti-Human IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Donkey Anti-Human IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution for specific applications. Use appropriate controls to ensure specificity and sensitivity.
Validation Images