Goat anti-BSA
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.99448-1mg | €27.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.99448-10mg | €1562.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.99448-100mg | €1562.50 |
| Catalog No | CAB.99448 |
|---|---|
| Product Name | Goat anti-BSA |
| Isotype | IgG |
| Calculated MW | 66kDa |
| Immunogen | BSA |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Bovine |
| Application | WB;ELISA |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300（PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Bovine Serum Albumin. Albumin is one of the most extensively utilized proteins in the medical industry. Its availability，inherent stability，and ligand binding ability make albumin an essential component in the production of many therapeutic drugs，vaccines and diagnostic tests. Albumin is a soluble，monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids，fatty acids，and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65，000. The human albumin gene is 16，961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product，proalbumin，is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
Protocol / Instructions
## Introduction
The Goat anti-BSA antibody is a versatile tool for detecting Bovine Serum Albumin (BSA) in various applications. This protocol outlines the procedures for using this antibody in Western Blotting (WB) and Enzyme-Linked Immunosorbent Assay (ELISA).
For Western Blotting, the Goat anti-BSA antibody can be used to detect BSA in protein samples. The following reagents and buffers are required: protein samples, SDS-PAGE gel, transfer buffer, blocking buffer, and washing buffer. Sample preparation involves separating the proteins by SDS-PAGE and transferring them to a membrane. The membrane is then blocked with blocking buffer to prevent non-specific binding. The Goat anti-BSA antibody is incubated with the membrane at a dilution of 1:200-1000, followed by washing with washing buffer. Detection is achieved using a secondary antibody conjugated to an enzyme, such as horseradish peroxidase. Technical notes: optimize the antibody dilution and incubation time to achieve the best results.
For ELISA, the Goat anti-BSA antibody can be used to detect BSA in solution. The following reagents and buffers are required: BSA standards, sample diluent, coating buffer, blocking buffer, and washing buffer. Sample preparation involves coating the wells with BSA standards or samples, followed by blocking with blocking buffer. The Goat anti-BSA antibody is incubated with the wells at a dilution of 1:1000-10000, followed by washing with washing buffer. Detection is achieved using a secondary antibody conjugated to an enzyme, such as horseradish peroxidase. Technical notes: optimize the antibody dilution and incubation time to achieve the best results.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images