Sephadex® G-50 Size Exclusion Resin

Sephadex G-50 is a size exclusion resin with a molecular weight exclusion limit of approximately 30,000 Da, making it suitable for separating molecules in the range of 1,500 to 30,000 Da. This resin is composed of dextran beads that are cross-linked with epichlorohydrin, resulting in a porous structure that allows for size-based separation of molecules. Sephadex G-50 is commonly used for desalting and buffer exchange of small to medium-sized proteins, as well as for separating peptides and other biomolecules. The resin is available in various particle sizes and can be used in a range of applications, including chromatography and gel filtration. Its high resolution and ability to separate molecules based on size make Sephadex G-50 a valuable tool in biochemistry and molecular biology research. It is also relatively stable and can be used over a wide range of pH and temperature conditions.