2,3,5-Triphenyltetrazolium chloride

2,3,5-Triphenyltetrazolium chloride, with the CAS number 298-96-4, is a chemical compound commonly utilized in various biochemical assays. It is a tetrazolium salt that undergoes reduction to formazan when it comes into contact with reducing agents, resulting in a color change that can be quantitatively measured. This property makes it a useful tool for assessing cellular respiration, viability, and metabolic activity in tissues and cells. The compound is often employed in histological and cytological studies to differentiate between viable and non-viable cells, as well as to detect specific enzymatic activities. Its applications extend to fields such as plant physiology, microbiology, and medical research, where it serves as a valuable indicator of cellular function and integrity. The reduction of 2,3,5-Triphenyltetrazolium chloride is typically measured spectrophotometrically, allowing for precise quantification of cellular activity.